a Mitotic control and POC5 RNAi U2OS cells treated with STLC for 18 h and stained for centrin, POC5 and DNA. b Control and HAUS6 RNAi treated as in (a) and stained for centrin, HAUS6 and DNA. c Percentage of control or POC5 RNAi U2OS cells as in (a) with ≥ 4 centrioles. Data points from 3 independent experiments, 239-337 cells per condition and experiment (p < 2.22e-16, generalized linear model with binomial distribution). d Percentage of control or HAUS6 RNAi U2OS cells as in (b) with ≥4 centrioles. Data points from 4 independent experiments, 232–335 cells per condition and experiment (p < 2.22e-16, generalized linear model with binomial distribution). e Percentage of control or HAUS6 RNAi U2OS cells with ≥4 centrioles treated as depicted with or without BI2536. Data points from 3 (0.5 h, 2 h, 24 h + BI2536) or 6 (24 h) independent experiments, 115-350 cells per condition and experiment (p (0.5 h) = 0.915; p (2 h) = 1.2442e-05, p (24 h) < 2.22e-16, p (24 h, BI2536) = 0.0054128, generalized linear model with binomial distribution). f Control and GCP4 RNAi U2OS cells treated and stained as indicated. g Percentage of cells as in (f) with ≥4 centrioles after 0.5 h or 24 h in mitosis. Data points from 4 independent experiments, 100-140 cells per condition and experiment (p (0.5 h) = 0.2723, p (24 h) < 2.22e-16, generalized linear model with binomial distribution). h Control and HAUS6 RNAi U2OS cells as in (a) and stained as indicated. i Percentage of cells as in (h) with ≥4 centrin foci or ≥2 centrobin foci. Data points from 3 independent experiments, 300-504 cells per condition and experiment (p (centrin) < 2.22e-16, p (centrobin) = 0.018855, generalized linear model with binomial distribution). Horizontal lines and error bars in (c,d,e,g,i) depict means and standard deviations, respectively. Scale bars, 10 µm (cells) or 1 µm (magnifications). ***p < 0.001; *p < 0.05; n.s., not significant. Source data are provided as a Source Data file.